Composite

Part:BBa_K3519006

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)


medium promoter-sulX

This part has the sulX gene (BBa_K3519000) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015).

Usage and Biology

For more information on previous characterisations and effects of overexpression of the sulX gene please visit the part BBa_K3519000.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 509
    Illegal AgeI site found at 637
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None